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Fast and flexible testing

On-farm pregnancy test with results in 5-20 minutes

Alertys OnFarm should find a place on all farms

Your farm, your timing.

You’re in control. Alertys OnFarm is a super-fast and reliable pregnancy diagnosis that you can carry out yourself, at a time to suit you. 


Consistently accurate and easy-to-use, while using just a few drops of blood from the cow, results are available between 5 and 20 minutes after testing, allowing you to make quick, informed decisions about individual animals.


Good for the farm

happy male farmer on cow farm around herd.jpeg

With accuracy rates of >99%¹, Alertys OnFarm is an efficient and easy-to-use pregnancy diagnosis.


Its minimal training requirement makes it ideal for more remote farms, for testing small groups of animals outside the normal timetable, or for those times when the Vet simply can’t be there.


Alertys OnFarm is accurate in detecting a pregnancy as early as 28 days after service. It’s a great new tool for both dairy and beef producers.

¹Kline, Adalaide C.; Walker, Julie; Andrews, Taylor N.; Zoca, Saulo Menegatti; Epperson, Kaitlin M.; Quail, Lacey K.; Ketchum, Jaclyn N.; Rich, Jerica J.J.; Rhoades, Jim R.; and Perry, George, "Comparison of Lateral Flow to Other Pregnancy Determination Methods in Order to Determine Accuracy of Pregnancy Status in Beef Cattle Pre and Postpartum" (2022). SDSU Beef Day 2022 Summary Publication. 1.

Good for the cow

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Just a few drops of blood on a lateral-flow test cassette. That’s all you need to complete the test.


No extra handling, no rectal exam, no need for a Vet to be present. Just an easy-to-read pregnancy diagnosis, within minutes.

It’s also risk-free – no need to worry that a mistimed intervention might cause a cow to abort.

Good for the Vet


Alertys OnFarm can be used as a 

pre-screening test, to help the Vet focus on the cows that need help.


Rather than perform a pregnancy diagnosis on the whole herd, Vets can focus on cows in need of attention: those that are sick, those that have aborted, those that aren’t getting in-calf.


Your Vet has more time available to spend on herd health plans: devising and refining strategies to improve fertility, minimise lameness, maximise milk production and optimise profitability.

All benefits at once

Identify non-pregnant cows any time, on-farm


Just a few drops of blood: all that's needed to run the test

No need to send in samples: tests are conducted on-farm

Use the test at your convenience, whenever you want

>99%¹ accuracy from day 28 post-AI. As accurate as veterinary advice

Results in minutes so you can take immediate action

Alertys OnFarm proud to win awards as the best innovation for farmers 

Alertys OnFarm kit tests
Innov Space 2022
SPACE innov space 2022
Inel d'or 2022
IDEXX representatives at INEL d'OR 2022

"The test is quick, 99% accurate and easy to use"

Edoardo Borgia

240 cows, Le Rogaie farm, Grosseto, Italy

“By using the test, the diagnosis can be brought forward to 28 days and this time interval on a possible negativity is essential and must be made up for. Another advantage of the test is ease of use."

Professor Les Anderson

PhD, University of Kentucky

“The Alertys OnFarm test will help us to identify cows that get pregnant early. It’ll improve our efficiency tenfold.”

Phil Trowbridge

Trowbridge Angus, Ghent, New York

The Alertys OnFarm Pregnancy Test for us has been an unbelievable time-saver because we can do it whenever we want.”

Amael Gattaux

90 cows, Earl du Bon air, France

"Alertys OnFarm comes with these benefits: early detection from days 28 post-AI and its fast speed. We were able to get the results quicker, which gave us a good indication of problem cows."

Are you considering trying Alertys OnFarm?

We work together with trusted partners and distributors to offer Alertys OnFarm pregnancy test kits to farmers all over the world.

Complete the form and we’ll email you further details about the test, as well as contacts of our local trusted partners.

That was it! Thank you for requesting more information about Alertys OnFarm. You are on your way to quick and accurate, on-farm pregnancy testing results in as little as five minutes.​ Our information pack should soon arrive in your inbox (although there is a small chance it might go to your junk or spam folder; please check that too!).

  • What is PAG and how do the Alertys tests detect it?
    ​PAG is shorthand for Pregnancy-Associated Glycoprotein. The PAG test which has been developed by IDEXX is called the Alertys Pregnancy Tests*. It is an enzyme-linked immunosorbent assay (ELISA) test. It has been developed to accurately detect PAG molecules in blood, serum or milk. It is validated to be used for pregnancy diagnosis in cattle, sheep, goats, water buffalo and bison. Unlike other chemical tests for pregnancy using progesterone as an indicator, the PAG test is very specific for pregnancy. *The Alertys Rapid Visual and OnFarm pregnancy tests can be run without ELISA instrumentation.  PAGs are detectable as early as 28 days after insemination, so it is recommended that the test can be used from this point and throughout the whole gestation period.
  • What lab equipment do I need to perform the Alertys Milk and Ruminant Pregnancy Test?
    For performing these tests, you should have a lab equipped for ELISA testing, including pipettes, pipette tips, distilled water, a 96-well plate reader, a microplate washer, and a plate shaker/incubator. All details can be found in the kit inserts.
  • What is the difference between PAG and Progesterone?
    Progesterone is a hormone that occurs with peaks and valleys during the normal reproductive cycle and is not pregnancy specific. The Alertys pregnancy tests detect pregnancy-specific proteins called pregnancy-associated glycoproteins (PAGs). These specific proteins are expressed in the embryonic regions of the placenta and detected in maternal fluids such as blood and milk.
  • What are the differences among IDEXX Alertys products?
    The Alertys Milk Pregnancy Test uses milk samples collected during routine milk recording or collected from self-sampling pots, which will be most suitable for dairy farms. The test cannot be performed on-farm as specialist laboratory equipment is required. The Alerty Ruminant Pregnancy Test, which uses blood samples, on the other side, may be more convenient for beef herds. Again, the samples are tested in a lab as specialist equipment is required. For smaller-scale testing organisations, such as individual veterinary practices, the Alerty Rapid Visual Pregnancy Test can be used. A blood or serum sample is required, and the test can be completed in around 30 minutes. Some technical skill is required, and this isn’t a cow-side test, but there is no necessity for specialised laboratory equipment to observe the colour change to make a pregnancy diagnosis. Finally, there is an Alertys OnFarm Pregnancy Test, which was recently introduced in 2021. This lateral-flow style test can be used on farm and requires no technical expertise, other than to collect a blood sample (EDTA sample). A measured amount of blood is dropped onto the test cartridge, and a pregnancy diagnosis result is ready in a matter of minutes, not unlike a human home-pregnancy test kit. This is suitable as a cow-side test.
  • What is the return on investment from using Alertys tests?
    Open cows can have a major impact on profitability. Research shows that the average cost per open dairy cow is €5 a day*. These costs include the value of the milk the cow would have produced, the value of her calf, and other relevant factors. Dairy farms can optimise profitability by shortening the calving interval and reducing the number of days that cows are open. For beef farmers, shorten calving intervals with safe, ongoing confirmation of pregnancy status at critical stages, saves an estimated $77 per head** by identifying and removing sub-fertile cows early. * De Vries et al, Economics of improved reproductive performance in dairy cattle (Publication AN 156), Gainesville FL, University of Florida Institute of Food and Agriculture Science; 2005 ** Cook B, Biermacker JT, Childs D. The value of pregnancy testing beef cows. Paper presented at: 2007 Southern Agricultural Economics Association Annual Meeting; February 4–7, 2007; Mobile, Alabama.
  • Can all the Alertys tests tell anything else besides pregnancy?
    No, the test will provide a pregnant, open/non-pregnant or 're-check' result. It cannot tell the stage of gestation, the sex of the calf or if the cow is carrying multiple calves.
  • Why test more than once during gestation?
    During the normal gestation process of a cow, approximately 10-25% of pregnancies will be lost between conception and full-term due to unknown causes. Pregnancy testing at specific times throughout gestation helps improve reproductive efficiency by finding cows who have experienced pregnancy loss. It is recommended to test 2-3 times throughout gestation; first at 28-30 days post- breeding, again at 60 days post-breeding, and before dry off to be sure only pregnant cows are kept on farm.
  • Does PAG testing replace the need for a Vet?
    It is clear there is a strong case for early and accurate pregnancy diagnosis in dairy herds. The method used for pregnancy diagnosis is somewhat a matter of personal choice, depending on individual circumstances and convenience. Both transrectal ultrasound (TRUS) and PAG testing are reliable methods of early pregnancy diagnosis. Most dairy farms find that working closely with a progressive veterinary practice carries many benefits. This remains the case whether you choose to use the Vet for TRUS pregnancy diagnosis, or not. Of course, transrectal ultrasound can play a role in other areas of fertility management too, such as diagnosis of non-cycling or cystic cows. A mix-and-match approach can be a sensible way forwards for many herds, taking advantage of PAG pregnancy diagnosis for its timeliness, simplicity, and accuracy, whilst reducing the numbers of cows needing to be separated and presented at the routine vet visits. Some farms find this then frees up their Vet to focus on valuable tasks, such as fertility data analysis, training, and other beneficial work.
  • Is there an influence of breed on all the tests?
    No breed influence on test performance has been described.
  • Do antibiotics influence the PAG tests?
    IDEXX has no evidence to date to suggest that antibiotic treated milk has a negative effect on the test results.
  • After early embryonic losses or abortion, does the test still detect PAGs?
    Yes, PAGs will circulate in milk and blood for a certain period of time after embryonic loss or abortion. For early embryonic loss, we estimate PAGs will disappear within 6-10 days. In case of late term abortion PAGs may be present for a longer period of time (40-60 days). It should be noted that it can take up to 60 days for PAGs to drop below the test threshold post-calving.
  • I have a bull in my herd or use a bull at the end of my AI period. Can I still use PAG testing methods?
    You can still use one of the Alertys tests, you just need to apply some rules around testing. For example, test 50 days after the cow is put with bull (21 days for heat plus 28 days to earliest test), or test 28 days after the bull is removed from the herd. If the bull is running with the herd all the time, you may need to test monthly, BUT remember, the test will not tell you how far in calf the cow is. And a negative result does not mean not-pregnant if test is done less than 28 days since service.
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